Design of a Facile Glutathione Sensing Method on the Basis of Peroxidase-like activity of ۲DTMD/MNP nanocomposite

Due to the pivotal role of glutathione (GSH) in key biological processes, including intracellular signal transduction, detoxification, preservation of proteins structure, regulation of gene expression, and also the difference of its level between normal and cancer cells, development of a rapid, inexpensive and accurate sensing method is of great importance. Here, on the basis of the inhibition effect of GSH on the enzyme-like activity of peroxidase mimetic ۲DTMD/MNP nanocamposite, a facile colorimetric method for glutathione sensing was established. After optimizing the assay condition, peroxidase-like activity of the nanozyme was detected in the presence of different concentrations of glutathione. In this sensing system, GSH can effectively inhibit the oxidation of TMB substrate in the presence of H۲O۲. The absorbance intensity at ۶۵۲ nm was descended along with the increased concentration of GSH, as the reaction mixture turn from blue to colorless at ۱۱۲.۵ μM of GSH, detecting by naked eye. These findings offer an appropriate platform for colorimetric detection of GSH in a linear range of ۰.۰۲ μM to ۰.۱۷۵ μM. Regarding the interfering effect of some other biological molecules such as aminoacids and sugars in glutathione sensing system, the selectivity test is underway.

کلیدواژه ها:

Peroxidase mimetic ، Nanozym ، Colorimetric method