Effect of inhibition of miR-21 on lncRNA of tumor suppressor gene role in MCF-7 cells

MiR-21 upregulates and this molecule as oncomiR causes survival of cancerous cell and decrease of apoptosis. For miR-21, many macromolecules have been found as targets like components of signaling pathways, DNA repair system and BCL-2 family influencing on many processes such as proliferation, metastasis, and progress of cancer. Based on comprehensive bioinformatics studies: co-lncRNA, Enrichment analysis, Gene Ontology, KEGG, two lncRNAs with two different tumor suppressor and oncogenic roles were considered. In order to inhibition of miR-21, the cells were transfected by anti-miR-21 and due to more confirmation of induced apoptosis, expression of genes non-coding RNAs and expression of two important apoptotic molecules BAX, and P53 were scrutinized by RT-qPCR. Assessment of apoptosis using MTT was done as well. Results showed that after transfection, miR-21 was down-regulated while lncRNA of tumor suppressor role was up-regulated. In this case, negative relationship between miR-21 and lncRNA of tumor suppressor was confirmed.

کلیدواژه ها:

MiR-21 ، lncRNA ، apoptosis ، breast Cancer