ازدیاد غیرجنسی درخت کنار ‭(Ziziphus spina- christi Willd ) ‬به طریق کشت بافت و سلول و تکثیر بوسیله روش های معمول و تعیین بهترین روش با توجه به جنبه های اقتصادی

Christ s thorn (Ziziphus spina-christi Willd.) from Rhamnaceae family is a native trees of southern tropical zone of Iran, and is more important for agroforestry and distribution of plant cover, because of resistance to drought and also, because of having tannin and saponin in different organs, and edible fruit, that is respondent to people s relish, and it can be introduce as a multipurpose tree. Therefore, cloning of this tree species is too important. For this reason, different types of asexual propagation were surveyed, that between them, stem cutting, air-layering budding and in vitro (single-node culture) methods were succeeded. In stem cutting, factors as diameter of cutting, season of cultivation, humidity supply, leafy or leafless cuttings, maturity and juvenility of cuttings, medium and auxin plant growth regulators were surveyed. The results were shown that cuttings with more than 8 mm in diameter, that were cultivated in Farvardin 1 to 20(March 21 to April 9) in out-door condition were succeeded. The other cases were no significance. In air-layering method, it is essential to use of growth regulator treatment, and the best results were obtained by 15000 ppm NAA and 20000 ppm IBA. In grafting method, budding practices in Mehr 16-30 ( October 7-22) were more successful in Khuzestan condition. In micropropagation, single-node culture system has the best results, and as the procedure, fifth to eighth node below the shoot apices during summer months (specially Mordad or July 23 to August 22) should be harvested and after sterilization with 7.5% solution of Ca(OCl)2, proper shooting treatment should be achieved in the hormone-free MS medium, and rooting treatment should be achieved with White s liquid medium pretreatment containing 25 mg/l IBA, and then establishment in semi-solid hormone-free White s medium containing 1 g/l activated charcoal. Meanwhile, the best condition of root for establishment in sand medium was obtained in 3 days after root initiation, with 85.7% successful. Key words: Christ s thorn (Ziziphus spina-christi Willd.), Asexual propagation, Stem cutting, Air-layering, Budding, In vitro system (single-node method)

کلیدواژه ها:

کنار ، تکثیر غیرجنسی ، کشت بافت ، روش قلمه ساقه ، روش خوابانیدن هوایی ، روش پیوند جوانه (کوپیوند) ، روش درون شیشه ای (کوپیوند) ، موقعیت اقتصادی