بررسی مطالعه پاتولوژیک و ردیابی حضور ویروس در ارگان های خوکچه هندی بعد از تزریق تیپ ‭o ‬ویروس تب برفکی

In order to find out the duration of persistence of FMD virus in different organs of guinea pig after intra dermal rout of administration in plantar surface of leg as well as determining the histopathological lesions induced by the virus, at first, we adapted the virus to the guinea pig, by injecting the FMD virus to guinea pigs. After observations of generalization of the FMD virus and preparation of adapted virus, to guinea pig, other guinea pig were divided randomly into groups, each group consisted of animals. animal in each group injected by adapted virus and one guinea pig in all groups by injection of normal saline. On , , , and days post inoculation (PI), all the animals of one group selected and bleed for determining of anti bodies against FMD virus by serum neutralization method. Then the animal euthanized and samples of different organs consisted of heart lung liver spleen pancreas, retropharyngeal lymph node plantar epithelium inguinal lymph node and tongue epithelium were collected. One part of the each samples referred to department of pathology for histopathologic examination. Virus isolation, Elisa and PCR tests were performed on the other part of the tissue samples. Based on our study the most outstanding lesions due to FMDV were observed in plantar epithelium, lung, tongue epithelium and spleen, respectively. Intracellular spongiosis, vacuolization and pyknosis of cells in spinous layer, intercellular edema in the spinous layer of Plantar and tongue epitheliums, increased size of interalveolar septa in the lung and decreased of white pulp in comparison with control animals were the most obvious lesions in histopathologic examination of the sampled tissue. The result of Sandwich Elisa tests showed that there was only low amount of virus in the spleen the on day PI. Subsequently presence of virus was detected in the heart, lung, pancreas, non-inoculated plantar epithelium on day PI. The quantity of the virus in non-inoculated plantar epithelium was higher than other organs. On day PI, presence of the virus was detected in most organs. However, there was no sign of presence of virus in any organs on and days PI. Totally the results of Elisa tests, presence of virus, was wellmatched with the lesions in plantar epithelium, lung, tongue epithelium and a little with the lesions in the spleen. Description of PCR assay result on days PI showed weak band getting from PCR product of spleen. Whereas, on day PI same bands were observed in Heart, Lung, Pancreas, and plantar epithelium. On day PI those band were observed in heart, lung, liver, pancreas, plantar epithelium. However, there was no band in any organs on days and PI. On days PI rising of antibodies titer, reason for generalization virus in guinea pig،s body, began to increase which was Continued until days PI and reached to peak on days PI. However, it was decreased on days and PI. Generally according to the results of present study, it is believed that the major sites of FMD virus persistence in guinea pig tissues are plantar epithelium, lung, and tongue epithelium respectively. The most increased level of virus persistence was on days and PI, respectively.

کلیدواژه ها:

آفتوویروس ، خوکچه های هندی ، آسیب شناسی ، روابط میزبان-بیماریزا