Markers of Oxidative Stress in Rat The Effect of Experimental Thyroid Dysfunction on Pancreas

Background and aim: The aim of the present study was to evaluate the effects of thyroid dysfunction on markers of oxidative stress in rat pancreas.Material and methods: Hypothyroidism and hyperthyroidism were, respectively, induced in rats via administration of propylthiouracil PTU and L-thyroxine sodium salt in drinking water for 45 days.Results: The activities of superoxide dismutase SOD , catalase CAT , glutathioen peroxidase GPx , glutathione reductase GR , glucose-6-phosphate dehydrogenase G6PD , xanthine oxidase XO , and nonenzymatic markers of oxidative stress including malondialdehyde MDA , protein carbonyl PC , reduced glutathione GSH , and total thiols T-SH were determined in the rat pancreas. In hyperthyroid rats, pancreatic CAT, SOD, GPx, GR, XO, G6PD activities were increased compared with those in hypothyroid and control groups. There were no differences in activities of antioxidant enzymes between hypothyroid and control rats. Pancreatic MDA and PC in hyperthyroid rats increased compared with hypothyroid and the control animals. Whereas, hyperthyroid rats had decreased levels of tissue GSH and T-SH compared with hypothyroid and the control groups.The findings showed that only GSH level has decreased significantly in the hypothyroid group compared with control groups.Conclusion: our results showed that experimental hyperthyroidism induces oxidative stress in pancreas of rats, but hypothyroidism has no major impact on oxidative stress markers.

کلیدواژه ها:

hyperthyroid ، hypothyroid ، antioxidant enzymes ، oxidative stress markers