Physical Properties and Stability of Plasmid DNA-Loaded Chitosan-TPP Nanoparticle
محل انتشار: فصلنامه روشهای تصفیه محیط، دوره: 7، شماره: 3
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 561
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شناسه ملی سند علمی:
JR_JETT-7-3_024
تاریخ نمایه سازی: 6 اسفند 1398
چکیده مقاله:
Chitosan (CS) is a biodegradable natural polymer that has shown potential for gene delivery. Although a number of in vitro studies showed that chitosan and its derivatives have emerged as promising vehicles for efficient non-viral gene and plasmid DNA (pDNA) vaccine delivery, the stability of chitosan/plasmid nanoparticle remain insufficient. In the present study, ionically crosslinked chitosan nanoparticles were formulated with plasmid DNA using the ionic gelation technique with sodium tripolyphospate (TPP) as a crosslinking agent. We investigate the stability of chitosan/pDNA nanoparticles which was synthesized by this method. Optimization study showed that chitosan to TPP ratios of 1:0.4(w/w) results in the reproducible formation of nanoparticles with good production yields. SEM and DLS analyses revealed a circular shape of the CS/TPP nanoparticles with an average size diameter of 173 nm. The zeta potential of the nanoparticles was + 10.8 mv. In vitro study of pDNA release from CS/TPP nanoparticles revealed no DNA release following incubation of chitosan/pDNA nanoparticles for up to 1 month, in mediums of PBS and acetic acid at pH 4 and pH 7.4. According to the results, ionically crosslinked CS/TPP nanoparticles have the potential to be used as a biocompatible non-viral gene delivery system with strong stability.
کلیدواژه ها:
نویسندگان
S. R. Naeimi Torshizi
Department of cellular and molecular, Nour Danesh Institute of Higher Education, Mymeh, Isfahan
H Ofoghi
Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran
A Jangjou
Department of Emergency Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
S. Taghizadeh
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
M Kianirad
Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran