Anti-Biofilm Activity of Punica granatum, Ricinus communis,and Allium sativum Plant Extracts on Streptococcus mutans
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 297
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شناسه ملی سند علمی:
JR_IEM-4-2_005
تاریخ نمایه سازی: 3 اسفند 1398
چکیده مقاله:
Aims Streptococcus mutans (S. mutans) is part of human oral cavity microbiome and is known to be responsible of dental caries. The aim of this study was to evaluate the inhibitory effects of Punica granatum, Ricinus communis, and Allium sativum extracts on biofilm formation caused by S. mutans. Materials & Methods In this experimental study, the biofilm formation was carried out by broth dilution method with glucose -supplemented Tryptic Soy Agar (TSB) in 96-well microtiter plates. Seven serial dilutions from the aqueous extracts of the Punica granatum, Ricinus communis, and Allium sativum were prepared. Then, a suspension of S. mutans was added to the wells. The anti-biofilm effects of the extracts and turbidity were measured by an ELISA reader apparatus at OD492nm. Experiments were completed in triplicate. Findings Ricinus communis was more active on S. mutans than other extracts. In comparison with others, the mean OD obtained in the presence of a concentration of 50mg of the plant extract (OD=0.083) was close to the negative control (OD=0.068). This plant was effective in higher concentrations (50, 25, 12.5 and 6.25mg/ml). Allium sativum extract has a moderate effect on S. mutans. The lowest activity belonged to Punica granatum extract. Conclusion The extract of Ricinus communis has strong anti-biofilm activity against Streptococcus mutans, when compared to other extracts, Allium sativum extract show moderate activity on the biofilm formation. Aqueous extract of Punica granatum peel isn’t very effective on S. mutans
کلیدواژه ها:
نویسندگان
s Shams
Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
a Mehdipour
Pediatric Dentistry Department, Dental Faculty, Qom University of Medical Sciences, Qom, Iran
s Kermani
Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
h Ghorbani
Student Research Committee, Qom University of Medical Sciences, Qom, Iran
s Ragolia
Molecular Medicine Department, University of Padova, Padova, Italy