The membrane mesenchymal stem cell derived conditioned medium attenuates focal cerebral ischemia/ reperfusion injury in rats

Background and Aim : Stroke is a cerebrovascular disorder that results in a high degree of mortality and morbidity around the world. Previous studies have shown that conditioned medium (CM) obtained from mesenchymal stem cells (MSCs) might exert neuroprotective effects against different neurodegenerative disorder. The present study was performed to investigate whether the amniotic mesenchymal stem cells-conditioned medium (AMSC-CM) can confer neuroprotection against focal cerebral ischemia/ reperfusion (I/R) reduction of blood–brain barrier (BBB) damage and reduce of apoptosis.Methods : A rat model of middle cerebral artery occlusion (MCAO) was created and the animals were divided into three groups including sham, MCAO and MCAO+ hAMSC-CM. The AMSC-CM at the dose of 1 μl was intravenously administrated to rats. Apoptosis and BBB damage , were assessed 24 h after reperfusion using TUNEL staining and Evans Blue (EB) uptake, respectively.Results : intravenously administration of AMSC-CM markedly reduced BBB damage compared to MCAO group (P < 0.05). Additionally, treatment with AMSC-CM significantly reduced apoptotic cell death compared to MCAO group (P < 0.05).Conclusion : Collectively, our results demonstrated that hAMSC-CM contributed to neuroprotection following ischemic stroke by reduction of BBB damage and apoptosis. These findings suggest the validity of AMSC-CM as a good therapeutic agent for acute stage stroke.

کلیدواژه ها:

cerebral ischemia/ reperfusion ، amniotic mesenchymal stem cells ، conditioned medium ، apoptotic cell death ، BBB damage