Differentiation of human breast-milk stem cells to neural stem cells

Background and Aim : There is a diverse population of stem cells in human breast milk that can be employed for therapeutic purposes as a non-invasive source of cells. The current study mainly was aimed to differentiate the breast milk stem cells (BMSCs) toward neural stem cells.Methods : the BMDSC were isolated from human breast milk the days1 to 4 after parturition and cultured in Dulbecco’s modified Eagle’s medium high glucose containing 20% knockout serum replacement (KOSR). Then, the cells were exposed to culture medium include 3% B27 and 2% N2, fibroblast growth factor (bFGF) 10 ng/mL, and endothelial growth factor (EGF) 20 ng/mL. The suspend neurospheres were incubated in differentiation medium containing 5% fetal bovine serum without any growth factors. Seven days after differentiation, express of glial fibrillary acidic protein (GFAP), microtubule associated protein (Map2), ?-tubulin III and Neun were evaluated using immunofluorescent.Results : The results showed BMSCs have the ability to be differentiated into neural cell lineages. According to the immunofluorescent analysis, the differentiated cells expressed neural markers as follow: GFAP 36.67%± 2.1, Map2 26%± 3, -tubulin III 19.77± 3.3 and Neun 24.33± 3.4.Conclusion : Based on the current study results, breast milk stem cells, as a novel source of stem cell can use for therapy in the neurodegenerative disease.

کلیدواژه ها:

breast milk stem cell ، differentiation ، neural stem cell