Antibacterial Susceptibility Evaluation of Novel Core-Shell nanoparticles against Gram Positive Pathogens Staphylococcus aureus and Streptococcus agalactiae

Background and ObjectiveIn recent years, bacteria have become highly resistant to antibiotic treatments. On the other hand, the speed of antibiotic production has been replaced with greater effect, although it is by no means responsive to the speed of increased bacterial resistance, and the urgent need for new approaches to dealing with bacterial infections. One of the ways to fight these microbial agents is to replace reliable antibacterial agents that different microbial species do not become resistant to these factors. Among these antimicrobial agents, metal nanoparticles that have been commonly studied in recent years can be studied. Staphylococcus aureus is one of the most important and effective pathogenic bacteria in hospital infections. Antibiotic resistance is one of the problems that are very important for the elimination of these bacteria. Therefore, nanomaterials can be used as a suitable material to control and eliminate this bacterium. The aim of present work is the evaluation of the antibacterial effect of PEI—Zncl4 and Fe3O4—sio2 nanocomplexes on the growth rate of gram-positive bacteria Staphylococcus aureus and Streptococcus agalactiae.Materials and MethodsIn this study, first, the 0.5 Mcfarland concentration of bacteria were exposed with the 1, 10 and 100 % w/v of nanocompounds and were incubated at 35 ºC overnight. Then the bacteria in the wells were cultured on the nutrient agar plated and the growth inhibition of this compounds was examined. Also, nanostructures were loaded on the blank discs in different concentrations. Disc containing nanomaterials put on the nutrient agar plates that were inoculated with a specific account of bacteria earlier. Then these plates were incubated at the 35 ºC for 18 hours. Inhibition zone diameter of each disc was measured as mean ± SD. Data were analyzed using Student t-test and p-value ≤ 0.05 was considered significant.ResultsOur finding reveals that the applied nanostructures in this study have a novel antibacterial activity against the gram-positive bacteria used. The higher and significant antibacterial effect was related to PEI—Zncl4 nanocomplex.ConclusionResults showed that the potential of using nanoparticle as an alternative to conventional antimicrobial agents that are currently used, and a perspective for application of these compounds with antibiotics to enhance antimicrobial activity

کلیدواژه ها:

Nanostructure ، Antibacterial activity ، Staphylococcus aureus ، Streptococcus agalctiae. Antibiotic resistance