The Protective Effect of Zataria Multiflora on the Embryotoxicity Induced by Bisphenol A in the Brain of Chicken Embryos

Bisphenol A (BPA), is one of the main industrial chemicals synthesized for various purposes. Materials and Methods: In the present study, thebrain tissue of chicken embryos was used to evaluate the embryotoxic effects of BPA and also the preventive effects of Zataria multiflora. To that end, fertile eggs were categorized into four groups (n=10). After four days, BPA (200 ppm) was injected into the air sacs of the eggsof the experimental groups. The Zataria multiflora (100 and 200 μg/egg) was administered into chick embryos 6 hours prior to administration of BPA. The control group simply was treated with olive oil. The eggs were placed in an incubator with a temperature of 37°C and a humidityof 63%. After 20 days, the embryos were beheaded and the brains were gathered for biochemical measurements. Results: The findings indicated that Zataria multiflora (200 μg/egg) significantly reversed the increased levels of MDA (p<0.05) and protein (p<0.001) in the brain ofBPA-exposed groupand also the decreased levels of total antioxidant and GSH as well as the activities of SOD and CAT in the brain of BPA-exposed group (p<0.01, p<0.05, p<0.05, p<0.05, respectively). Conclusion: Zataria multiflora reversed the toxic effects of BPAon the embryonic development stages of the brain via modulating oxidative stress.