Early Diagnosis of Parkinson’s Disease with Microarray Analysis Data

Parkinson’s disease (PD) is the second most common neurodegenerative disease, and it is a multifactorial disease with no definite diagnosticindex. The aim of this study is to construct a molecular network to find molecules that play important roles in the progression of PD with analysis the microarray data for early diagnosis and intervention. Materials and Methods: We downloaded two gene expression profiles(GSE54536 and GSE100054) from the Expression Omnibus (GEO) database to analyze possible markers.The Genes were analyzed with GEO2R. There were 1790 and 967 differentially expressed genes (DEGs) in GSE54536 and GSE100054 espectively. A total of 125 genes co-exist in the DEGs of the two data sets. Results: KEGG pathway analysis showed that 125 DEGs wereenriched in Aldosterone synthesis and secretion, Gap junctions, Platelet activation, Rap1 signaling pathway, and Estrogen signaling pathway. There were 20 hub genes among 125 DEGs analyzed by PPI that involved in Platelet activation, Inflammatory response, Innateimmune response, B cell receptor signaling, Stimulatory C-type lectin receptor signaling, Lipopolysaccharide response, Leukocyte migration, and Regulation of cell proliferation. Additionally, 42 differences in miRNAs were found in GSE100054. We constructed a miRNAmRNAregulatory network depicting interactions between the predicted genes and the 125 DEGs. 34 miRNA-mRNA pairs were obtained. We found GNAQ and TMTC2 were the most important mRNAs in the network analyzed by Cytoscape APP centiscape, and their degrees in centiscape2.2 were all 10. has-miR-142 was the most important miRNA (the highest degree is 4 in centiscape2.2), which forms miRNA-mRNA pairs withGNAQ, TMTC2, BEND2, and KYNU. Conclusions: This study provides data of potential biomarkers and therapeutic targets for PD diagnosis and treatment. Among them, hsa-miR-142 is a critical miRNA in the PD network, and may be involved in PD progression by regulating GNAQ, TMTC2, BEND2, and KYNU