Comparison of two Ovarian Stimulation Protocols on Developmental Competence of Ovum Pick Up Derived Jersey Oocytes

Background: This experiment was the first study in the world conducted to evaluate the effect (s) of two protocols of ovar-ian stimulation using FSH, prior to ovum pick up (OPU), on quantity and quality of retrieved oocytes and their subsequent developmental competence.Materials and Methods: The Jersey heifers (n=9) after estrous synchronization were randomly assigned into three experimen-tal groups: I) heifers receiving 225 mg FSH in two equal doses for one day (1-Day) II) heifers receiving 225 mg FSH in four decreasing doses for two days (2-Day), and III) heifers receiv-ing no FSH treatment (control).Results: There were no significant differences between groups in quantity and quality of recovered COCs per OPU session. The cleavage rate was significantly higher in 2-Day group com-pared to the other groups (83.6 ± 2.8 vs. 67.9 ± 3.3 and 74.4± 2.8; P= 0.001). The blastocyst rate in 2-Day group on day 7 (44.8 ± 4.1 vs. 22.7 ± 3.4 and 33.7 ± 3.4; P= 0.002) and day 8 (47.3 ± 4.3 vs. 26.7 ± 3.4 and 36.1±3.4; P=0.001) was signifi-cantly greater compared to other groups. No significant difference in transferable embryos was observed among groups. Conclusion: In conclusion, two days of ovarian stimulation, in comparison to one day stimulation, increased the quality of recovered COCs which was evidenced by their higher develop-mental competence.

کلیدواژه ها:

Ovum Pick Up ، Jersey ، Embryo ، Development