Effects of Biolozax-H on The Testis Structure and Pi-tuitary- Gonadal Hormones in Adult Rat following Treat-ment with Clinical Dose

Background: The activity of the renin-angiotensin system has an effect on oxidative stress in many tissues, including the tes-tes. Therefore, in this study, the biolozax-H drug, which has an effect on the spermatogenesis activity and Pituitary- Gonadal Hormones in male rats, is further investigated.Materials and Methods: Male adult rat (200-250 g) were di-vided into two groups of sixteen each. Control and biolozax-H treated group were administered 20 mg/kg biolozax-H for 45 days, respectively. The animals were sacrificed 45 days after starting treatment. The histological change on germinal epithe-lium, and Level of hormones was measured by Radioi Immuno Assay (RIA) method. Then, tissues were fixed in Buin s fixa-tive. Sections were cut into 5 μm thicknesses and stained with Hematoxylin and Eosin (H& E). The achieved results were ana-lyzed in SPSS software using t-test and ANOVA.Results: Count of spermatogenic, Sertoli and leydig cells significantly decreased in the experimental group in com-parison with the control and Control groups (P<0.05).also In the experimental group,titer of FSH, LH and testoster-one hormone decreased significantly (P<0.05) in comparison with Control.Conclusion: Regarding physiological role of Sertoli cells dur-ing spermatogenesis, reduction of FSH hormone may lead to negative effects on the sperm production and reproductive po-tential of male Rat.