Protective Effect of Misoprostol on Ibuprofen-In-duced Alteration of Sperm Parameters in Adult Male Mice

Background: Ibuprofen, a propionic acid derivate is a nonsteroidal anti-inflammatory drug (NSAID). In recent years sev-eral studies have been published on animal and human clini-cal experiments with NSAID, pointing to the deleterious effect of prostaglandins (PGs) on spermatogenesis. Misoprostol is a synthetic prostaglandin E1 methyl analogue indicated for the prevention of gastric ulcers induced by NSAIDs. The aim of the present study was to determine the protective effect of misoprostol on ibuprofen-induced alteration of sperm quality and quantity parameters in adult male mice.Materials and Methods: In this study, 80 adult male mice were divided into 8 groups including: control, ibuprofen treated group which received 6 mg/Kg/day ibuprofen, three misopros-tol treated groups which received 1, 10 and 100 µg/Kg/day mis-oprostol and three ibuprofen+misoprostol treated groups which received 6 g/Kg/day ibuprofen with 1, 10 and 100 µg/Kg/day misoprostol. All treatments were carried out for 40 consecutive days by oral gavage. At the end of experiment, animals were euthanized and their left epididymis was removed and dissected in Ham s F10 and incubated at 37°C. Total count, motility, vi-ability and morphology of sperm as well as tratozoospermia (TZI) and sperm DNA fragmentation index (SDFI) were as-sessed according to the WHO standard meth ods. Data were statistically analyzed by SPSS using one-way ANOVA test and Tukey s post-hoc.Results: Results showed that ibuprofen reduced total count, motility and viability of epididymal sperm and increased ab-normalities, TZI and SDFI significantly compared to control. It was also indicated that high dose of misoprostol could increase motility and viability of sperm and decreased TZI and SDFI significantly compared to control group. Our results revealed that all three dose of misoprostol could significantly increase total count, motility and viability of sperm and reduce sperm with abnormal morphology, TZI and SDFI compared to ibupro-fen treated group in a dose dependent manner.Conclusion: Based on our results it can be concluded that ad-ministration of misoprostol can improve quality and quantity indices of sperm and can be considered as a suitable protective strategy for improvement of male infertility or subfertility due to NSAIDs administration.

کلیدواژه ها:

Misoprostol ، Ibuprofen ، NSAID ، Sperm ، Male Infertility