The Effect of Glyphosate on the Fibrillation Mechanism of Bovine Serum Albumin

Glyphosate, the active ingredient of some commercial herbicides, has been the most widely used herbicide in worldwide agricultural crop productions. Glyphosate inhibits the biosynthesis of aromatic amino acids (i.e., phenylalanine, tyrosine, and tryptophan) and leads to several metabolic disturbances, including interruption of protein production, secondary product biosynthesis, and a general metabolic disruption of the phenylpropanoid pathway owing to reduction in the biosynthesis of aromatic amino acids. Protein aggregation is impacted by many factors including temperature, pH, and the presence of surfactants, electrolytes, and metal ions. Fibrillation generally develops under conditions in which the protein is in a partially destabilized or misfolded/unfolded statecompeting with the normal folding pathways.Bovine serum albumin is an important transport protein of the blood and its aggregation/fibrillation would adversely affect its transport ability leading to metabolic disorder. In this report the qualitative and quantitative aspects of the effect of glyphosateon heat induced aggregation/fibrillation of BSA at physiological pH (pH 7.4) have been studied employing a combination of ultraviolet-visible fluorescence spectroscopy, differential scanning calorimetry (DSC), dynamic light scattering (DLS) and atomic force microscopy (AFM).The objectives of this study were to examine the impacts of glyphosate applications on fibrillation pathway of bovine serum albumin and its impacts on metabolism of animals and end users‟ health, using meat as a food.

کلیدواژه ها:

Glyphosate ، Bovine serum albumin ، Fibrillation ، Aggregation