Heat shock protein gene expression in common carp (Cyprinus carpio) exposed to indoxacarb pesticide

Increasing the input of pollutants such as pesticides can has damaging effects on aquatic animals and ecosystems. In this investigation, 336 common carp (Cyprinus carpio) assigned to twelve 300L tanks with 28 fish 45.2-5.81 g per tank. The tanks were divided to 4 treatments which exposed to different sub-lethal concentrations of indoxacarb (0, 0.75, 1.5 and 3 ppm) during 21 days. HSP70 gene expression was studied by quantitative RT-PCR after cloning and sequencing of HSP70 gene from gill, kidney and liver of the fish. Results showed that the relative expression of HSP70 in the kidney of the indoxacarb-treated fish was significantly higher than that of the control group and the 3 ppm has the lowest gene expression among the pesticide-treated fish. HSP70 gene expression in liver of the fish exposed to 1.5 and 3 ppm of indoxacarb was significantly higher than that of those exposed to 0 and 0.75 ppm indoxacarb. HSP70 gene expression was induced in the gill of fish were exposed to 1.5 and 3 ppm of indoxacarb, while no change was observed for 0.75 ppm treated group. Meanwhile, the HSP70 gene expression of the fish treated with 3ppm was higher than those exposed to 1.5 ppm indoxacarb. The present results suggest that indoxacarb as an environmental stressor is able to up-regulate HSP70 gene expression in different carp organs, which is indicator of inflammation, osmotic and oxidative stress. Such adverse effects reduce the fish health and survivorship in natural environment.

کلیدواژه ها:

Indoxacarb ، Gene expression ، HSP70 ، Common carp ، Pesticide