Effect of drought stress on total protein content and antioxidant enzymes activity in Lathyrus Sativus at germination stage

The problem of drought and water scarcity in Iran has always been one of the greatest challenges in an agricultural industry affecting the production and yield of plants. In this research, the resistant cultivar of grass pea was treated with two mild and severe stresses (50% and 25% field capacity). Finally, in the four-leaf stage, total protein content and activity of oxidative enzymes in two leaf and stem samples were measured and compared to normal irrigation conditions (100% field capacity). The results showed that protein content in each leaf part increased considerably, but there was no significant difference in the stem. Evaluation of defense mechanisms by the activity of antioxidant enzymes showed that in the leaf sample and 50% field capacity treatment, the activity of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POX) and ascorbate peroxidase (APX) enhanced, while with increasing stress, the activity of these enzymes was reduced. The stem part, catalase activity raised in both drought stress treatments, while with increasing drought stress, the activity of the other three enzymes decreased significantly. The results showed that between four antioxidant enzymes, peroxidase enzyme had the highest activity in two parts of leaves and stems in the germination stage.

کلیدواژه ها:

Lathyrus sativus ، Catalase ، Peroxidase ، Ascorbate peroxidase ، Drought stress