The effect of sodium nitroprusside on some physiological and biochemical properties of canola (Brassica napus L.) under lead stress

In this research, the interactive effects of lead and sodium nitroprusside (SNP), a donor of nitric oxide (NO) on spring canola plants cv. PF were examined. 21-day-old canola plants in Hoagland s nutrient solution were treated with different concentrations of Pb(NO3)2 (0, 250, 500 μM) and SNP (0, 200 μM) for 10 days. Treatment with Pb decreased dry weight and Fe content of roots and shoots and reduced chlorophyll content in leaves. In Pb-treated plants, the activity of antioxidant enzymes such as catalase, guaiacol peroxidase, and ascorbate peroxidase significantly decreased in leaves, while, the level of lipid peroxidation increased. The results indicated that Pb caused an oxidative stress in canola plants. The results of Pb content assay in roots and shoots of plants showed that application of SNP reduced root-to-shoot translocation of Pb in Pb-stressed plants. Moreover, adding of SNP in the nutrient solution of Pb-treated plants increased Fe content of roots and shoots and chlorophyll content of leaves and improved the growth of plants. SNP also increased the activities of the antioxidant enzymes and decreased the level of lipid peroxidation in leaves of Pb-treated plants. Consequently, SNP markedly reduced Pb induced oxidative damage on canola plants.

کلیدواژه ها:

Lead ، Oxidative stress ، Nitric oxide ، Canola