Effect of salicylic acid on phenolic compounds content and antioxidant activity of Salvia Leriifolia Benth. in vitro

Norouzak (Salvia leriifolia Benth.) is a species of the Lamiaceae family which has anti-inflammatory, antiinflammatory, anti-diabetes, and anti-oxidant properties. Phenolic compounds, such as flavonoids, are secondary vegetable metabolites with very high antioxidant ability. In order to increase the production of secondary metabolites in tissue culture, treatments with biotic and abiotic elicitors and the addition of precursors are considered in plant cell cultures. The purpose of this study is to investigate the effect of various concentrations of salicylic acid (10, 50, and 100 mM) on the content of secondary metabolites and the antioxidant activity of the Norouzak plant in vitro.Thus, the contents of phenol and flavonoids are measured by Folin-cicoalteu and aluminum chloride, respectively. Also, the antioxidant activity is evaluated using DPPH method. The results showed that total phenol content, flavonoids, and antioxidant activity increased significantly than control so that the highest contents were observed in 50 mM salicylic acid. Therefore, by optimizing the concentration of acid salicylic acid, the antioxidant properties of S. leriifolia can be increased

کلیدواژه ها:

Salicylic acid ، Phenolic compounds ، Antioxidant activity ، Salvia Leriifolia Benth