Evaluation of the frequency of class I integron gene and antibiotic resistance pattern in Escherichia coli isolated from patients with urinary tract infections in Imam KhomeiniHospital in Sarab, Iran

Urinary tract infections are the second most common cause of infection in the body of human. Among the causes of urinary tract infection, E. coli is the most common causative agent of urinary tract infection (UTI), and UTI-induced antibiotic resistant E. coli isolates are increasing. Treatment of urinary tract infections due to antibiotic resistance in agents is getting difficult every day. Integrons are genetically mobile agents that promote the spread of antibiotic resistance genes among bacteria. The purpose of this study is to determine the antibiotic resistance pattern, to determine the prevalence of class I integrons and to determine its association with resistance to antibiotics in isolated Escherichia coli isolated from urinary tract infections in Imam Khomeini Hospitalin Sarab. Among 105 positive urine culture samples of patients with urinary tract infection from patients referred to Imam Khomeini Hospital in Sarab, 81 samples of Escherichia coli were isolated and determined by routine biochemical and microbiological tests. Antimicrobial resistance pattern of the samples was determined by the diffusion method in comparison with the antibiotics Ceftazidime,Imipenem,Trimethoprim, Ampicillin, Gentamicin, Ceftriaxone, Nalidixic Acid, and Chloramphenicol. After extraction of bacterial DNA by boiling and freezing method, the frequency of Integron Class I gene in E. coli isolated from urinary tract infection was determined using PCR and specific primers. In isolated samples of patients, the highest antibiotic resistance was seen to trimethoprim (85%) and nalidixic acid (74%) and the least resistance to gentamicin (11%). All of isolated Escherichia coli was sensitive to Imipenem and showed sensitivity to gentamicin (89%) and chloramphenicol (85%). The frequency of class I integrin gene was observed in 67 isolated Escherichia coli samples (82.7%). According to the findings of this study,it has the necessity to use the best treatment against urinary tract infections and considering the results of this study, it is advisable to use fewer antibiotics of Trimethoprim and Nalidixic acid in the primary treatment of urinary tract infections. The identification of the genes involved in the development of these resistances is one of the main needs of the study in the treatment of infections and class I integrons rate in Escherichia coli in the study is high, which may have a role in facilitating the spread of multiple drug resistance Strains.