Comparison of TCEB3 gene expression between breast cancer tissues and the adjacent non-tumor tissues

As the most common cancer among women in the world, breast cancer needs more to determine biomarkers for diagnosis and prognosis of it. Elongin A (ELOA or TCEB3) is one of the subunits of RNA polymerase II that potently activate the rate of it s transcription elongation. Elongin is a multimeric elongation factor comprising three subunits, Elongins A, B, and C. Here we aimed at evaluation of elongin A or TCEB3 expression in breast cancer. So, total RNA was extracted from twenty-five pairs of breast tumor tissues and their marginal normal tissues using RNX-plus reagent and also their cDNA was synthesized by PrimeScriptTM RT reagent kit(Takara) according to the manufacture s instructions. Expression of this target gene was evaluated by quantitative RT-PCR. In this study, GAPDH was used as an nternal control gene. Results showed significantly overexpression in tumoral tissues compared to the paired non-tumoral samples. Moreover, our findings showed that there was sa tatistical significant correlation between clinical-pathological features of tumors and ethe xpression level of TCEB3. TCEB3 expression was upregulated in ER+, PR+ and her2+breast cancer subtypes.

کلیدواژه ها:

Breast cancer ، TCEB3 ، q-RT-PCR ، Overexpression