Extraction of saponins fromTribulus terrestris and evaluation of its effects on human serum albumin (HSA) structure by UVvisibleandFT- IR spectroscopies

Tribulus terrestris is a medicinal plant, which widely distributed around the world. Saponins from this plant are responsible for its biological activities. Saponins as secondary metabolites that are found in many plants and some animals are high molecular weight glycosides, consisting of a sugar moiety linked to a triterpene or steroid aglycone.Saponins have cytotoxic, pro-apoptosis and antimetastatic effects on cancer cells. Furthermore, the total saponins extracted from the plant have beneficial effects on various ailments such as urinary tract infections, inflammations, leucorrhea, edema, and ascites.In this research after extraction of Tribulus terrestrisfractions, saponins were isolated by vacuum liquid chromatography (VLC) and thin layer chromatography (TLC). Then the effects of saponins were studied on the structure of human serum albumin (HSA) by UV-Visible and FT-IR spectroscopies. The results showed that Tribulus terrestris saponins can disrupt the HSA structure and can interact through the C=O and C-N groupsof the polypeptide chain. These results provided valuable clues to the pharmacokinetics and the pharmacologic activities of Tribulus terrestrissaponins

کلیدواژه ها:

Tribulus terrestris ، Human Serum Albumin (HSA) ، Saponins ، Spectroscopy