Role of β-diketonate group of curcumin on bilateral actions of it in presencedifferent concentrations of catalase

Curcumin as food additive extensively is being used around the world daily. In the present study, we investigated the role of β-diketonate group of curcumin on its actions in presence different concentrations of catalase using different spectroscopic and computational methods. Experimental and theoretical studies revealed that by rising concentrations of catalase, the enzymes were being protected by interaction other catalases via β-sheet domains and increases their stabilities. Following that, curcumin couldn’t inhibit catalase in higher concentrations, while in lower concentrations of catalase, curcumin can strongly inhibit this enzyme. However, by changing characteristic and structure of β-diketonate group of curcumin with binding Mn ion, curcumin lost these bilateral actions in presence different limited concentrations of catalase. It is suggested that interactions of catalases in higher concentrations prevented binding curcumin to the enzyme appropriately, while in lower concentrations of catalase, they cannot preserve themselves from toxicity effects of curcumin

کلیدواژه ها:

Catalase ، Curcumin ، β-diketonate group ، Inhibition ، Activation