Investigation of the possibility of stimulating the growth of single-cell yeasts using the combination of nanoparticles, L-carnitine and HUFA

Several studies have shown that nanoparticles can increase cell differentiation or enhance cell growth at specific concentrations. While nanoparticles inhibit cell growth in higher or even lower concentrations. Due to the importance of the application of single cell yeasts, the problem in the cultivation of some species and strains of yeast, this study was able to stimulate the growth of yeast cells using these materials. Therefore, Saccharomyces service yeast was grown under the influence of these materials in single and combination treatments. The yeast count was performed after 48 hours incubation period. The yeasts counting results showed that the highest concentrations compared to control revealed 82% increase in TiO2 treatment, 72% in HUFA with lecithin treatment and 40% in treatment L-carnitine. While the combined treatment with L-carnitine and nanoparticles increase by only 24%. Several studies have shown that nanoparticles can enhance cell differentiation or proliferation. In comparing the amount of biomass produced for different treatments, as well as the highest biomass was obtained in the same treatment compared to control. The study showed that the use of L-carnitine could differ the metabolism of fatty acids in yeast which alter culture conditions. The results of this study showed that providing to use of Nanoparticles and L-carnitine could increase of the yeast biomass production up to two folds, which in term of the cost of materials used and their values are highly functional

کلیدواژه ها:

Nanoparticles ، TiO2 ، Lecitin ، L-carnitine ، HUFA