Isolation of chrysogen parasitic bacteria from soils in Shiraz petrochemical complex and determination of chrysan remnants by them

Environmental pollution is one of the most important problems facing the world today. These contaminations occur as a result of the absorption and accumulation of chemicals in the food chain and the damage to and the damage to the plants and living organisms in that climate. Chrysene is a four-ring aromatic compound and one of the environmental threats. In this research, the decaying bacteria of chrysogen were isolated from the soils of Shiraz petrochemical region, and then the decompositionof chrysogen was studied under laboratory and kinetic conditions. At first, the specimens were incubated for 7 days at a temperature of 32°C after chilling in a basicmineral medium containing chrysogen. After separation and differential chemical testing, the MIC was used to isolate superior strains. Then, the strains were evaluated for growth kinematics and chromatographic analyzes, which were analyzed by chromatography using gas chromatography. At the end of the molecular tests, 16srRNAs were sequenced to identify the superior strains. Society and Sample: Shiraz petrochemical company and samples of contaminated soils in this region. Sampling method: A random sample was taken from several surface grafting points. Out of 23 bacterial species, two isolated bacteria, Pseudomonas putida, and Micrococcus luteuswere grown in the shortest possible time, and Pseudomonas putida chromatography analysis showed that in the course of 10 days, the bacterium decomposed 85% of 0.1% chrysene slowly. In this experiment, it was determined that two bacteria Pseudomonas putida and Micrococcus luteus are two major degenerators of Chrysogen, and these two bacteria can be tested for the rest of the multi-ring articular hydrocarbons