Investigation of RKIP tumor suppressor gene expression in triple-negative breast cancer cells under microgravity conditions

Microgravity is considered to be the main source of space flight shock that affects cells and tissues and has an adverse effect on the health of astronauts. Changes in gravity as experienced by astronauts during space travel lead to changes in the physiology and even the morphology of the cells in the culture medium. In some studies, it has been observed that changes in gravity reduce the growth of some cancer cells and alter the expression of some genes and cellular functions. RKIP protein is a modulator of kinase activity and a cell balancing agent that also acts as a metastatic inhibitor in a variety of solid tumors, including breast cancer. RKIP expression in advanced tumors is reduced and its increase reduces the invasive potential of cancer cells without affecting their growth. In this study, we have cultivated triple-negative MDA-MB-231 breast cancer cell line on a clinostat device in order to the investigation of the RKIP gene expression changes under simulated microgravity conditions. Our results indicate that weightlessness changes the expression levels of RKIP gene. In general, our findings would have created a new light on the effects of microgravity on cell biology and human health.

کلیدواژه ها:

microgravity ، RKIP protein ، MDA-MB-231 cell line