in-silico analysis of potential role of miR137 in patients with Schizophrenia

Background: Schizophrenia is a complex genetic disease and characterized by affective, cognitive, neuromorphological, and molecular abnormalities that may have a neurodevelopmental origin. MicroRNAs (miRNAs) are critical to neurodevelopment and adult neuronal processes by modulating the activity of multiple genes within biological networks. Noncoding variants in the human MIR137 gene locus increase schizophrenia risk with genome-wide significance.MiR137 as a brain-enriched microRNA, plays important roles in regulating embryonic neural stem cells (NSCs) fate determination, neuronal proliferation and differentiation, and synaptic maturation. Methods: To determine the gene targets of the differentially expressed miRNAs, we used three of the leading miRNA target prediction algorithms: mirwalk2, PicTar, and TargetScan. Mirwalk2 found the miRNA-target genes in the Carcinogenesis pathway and verified by KEGG pathways. KEGG pathway is derived from Kanehisa Lab, Kyoto University, Japan. This analysis is used to determine the position and role of this MIR137 in controling carcinogenesis pathway. The position and function of genes in a pathway are crucial for recognizing the mechanism of intervention or regulation of MIR137 in carcinogenesis.Results: Among the 638 predicted targets of mir137 with 7, 8 and 9 score. Our data manifested KEGG signaling pathways pathway in cancer and Schizophrenia as the most statistical relevant pathways with mir137 targetome. Several genes were identified that probably indicate a role for sustained Schizophrenia is a complex genetic disease. This is predicted that critical mRNAs in cholinergic synapse pathway.Conclusions: Our data suggests that miR137 acts as a regulator molecule by targeting some important mRNAs which are necessary in cholinergic synapse pathway, so it could be used as a prognostic biomarker in Schizophrenia.

کلیدواژه ها:

Schizophrenia ، miR137 ، biomarker