Evalition of mir-10a in CLL Patients and Healthy People using RT-qPCR

Background: Chronic Lymphocytic Leukemia (CLL) is the most common human leukemia occurring as indolent or aggressive form. MicroRNAs are small non-coding RNAs involved in several cellular processes and expressed in a tissue-specific manner. In CLL, microRNAs can function as oncogenes or tumor suppressors. The aim of the present study was to compare the expression level of mir-10a in normal and neoplastic samples from CLL patients by RT-qPCR.Methods: The number of 15 blood samples from patients with CLL and 15 blood samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-10a expression level was calculated using Livak method. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated that miR-10a down-regulation was significantly correlated with CLL cancer clinic pathological features. Expression level of mir-10a was 7.43% in CLL patients and 92.57% in healthy group (p value=0.001).Conclusion: Due to the previous reports, mir-10a act as tumor suppressor in CLL, which could be used as biomarker for CLL diagnosis. In this study we propose that miR-10a is a potential diagnostic marker and therapeutic target of CLL.

کلیدواژه ها:

Chronic lymphocytic leukemia ، microRN