A Sensitive Electrochemical DNA Biosensor for Anticancer Drug TopotecanBased on Graphene Carbon Paste Electrode

A main anti-cancer medicine is topotecan (TPT), which is a water-soluble derivative ofcamptothecin. TPT is so effective for treatment of human ovarian cancer, cervical and smallcell lung cancer [1].Some methods have been applied to determine topotecan and only a fewvoltammetric methods was used to study the electrochemical behavior of topotecan. The studyof interactions between drug and DNA is of present overall interest and importance, particularlyto design novel DNA-targeted medicines. Among the methods used to study the interactionbetween DNA and drug, electrochemical techniques are particularly promising because of theirhigh sensitivity and selectivity along with reasonably low costs [2]. Graphene is a low-costsubstance with excellent mechanical, electrical and thermal features and it has been recentlyconsidered considerably. Owing to mentioned features, graphene is a good alternative for usingin electrochemical biosensor [3].In current research, the interaction of topotecan with DNA isinvestigated with a graphene paste electrode and in solution phase via DPV. Based on theinteraction between dsDNA and topotecan, topotecan was determined by a simple, fast, reliable,and sensitive DNA biosensor. After optimizing the main experimental parameters, This DNAcoatedGPE was fabricated to evaluate its performance analytically. A linear dynamic range of7.0×10-7– 9.0×10-5 M was obtained for topotecan. Ultimately, the proposed biosensor wasfound to be capable of successfully analyzing human serum and urine samples.

کلیدواژه ها:

topotecan ، Drug-DNA interaction ، Graphene