IMMUNOLOGICAL AND PATHOLOGICAL CHANGES IN MICE TOWARDS INFECTION BY PASTEURELLA MULTOCIDA CULTURED IN HIGH AND LOW IRON MEDIA

Background and Aim:Pasteurella multocida is an important organism that is in charge of upper respiratory tract in human and animals. It has known that growth of this organism at different levels of Iron could influence the pathogenicity of the organism. Present study attempts to evaluate any changes in blood parameters and visceral organs of mice by cultivation of native Pasteurella multocida isolates from south of IranMethods:P.multocida was cultured on BHI medium and on the same medium supplemented with bipyridyl and BHI supplemented with Iron. Active and inactivated antigens were prepared from all medium and mice were injected and immunized two weeks apart, respectivelyResults:Blood samples were collected to evaluate the antibody against P.multocida. Tissue sections from euthanized mice were also prepared for pathological studies. There were high distraction and necrosis in lung, heart, kidney and spleen in Iron rich. Anti P.multocida antibodies titer did not show significant differences between groupsConclusion:It seems that Iron depletion would reduce growth and pathogenicity of pasteurella multocida while such condition would not affect its immunogenicity