GRAPHITIC CARBON NITRIDE NANOPARTICLE AS A SAFE AND EFFICIENT PHOTOSENSITIZER FOR PHOTODYNAMIC THERAPY OF METHICILLIN RESISTANT-STAPHYLOCOCCUS AUREUS

Background and Aim:The antibiofilm efficacy of PDT as a promising therapeutic option in the management of biofilm-associated infections can be improved by nanoplatform-based photosensitizers. Certain nanoscale materials such as graphitic carbon nitride (g-C3N4) have the ability to generate reactive oxygen species (ROS), due to their unique optical absorption properties, so allowing them to behave as photosensitizers. The aim of this work was to investigate the in vitro antibiofilm photodynamic efficacy of g-C3N4 nanoparticles against methicillin resistant-Staphylococcus aureus (MRSA).Methods:Three-dimensional (3D) nanostructures of g-C3N4 were successfully synthesized by one-pot thermal polymerization of dicyandiamide under nitrogen atmosphere. The biofilms were incubated with g-C3N4 nanoparticles and then irradiated with a white light-emitting diode (LED) light (emission range 400-800 nm). Also, we have investigated the in vitro cytotoxicity and phototoxicity of g-C3N4 nanoparticle on human dermal fibroblasts.Results:Atomic force microscopy (AFM) measurements confirmed the nanometric size of the prepared g-C3N4 particles. Graphitic carbon nitride mediated-PDT showed significant photoinactivation against 24h-old biofilm of MRSA (> 3.8 log10 CFU reduction). At the same experimental conditions, only 29.2% of the fibroblasts were photo-inactivated.Conclusion:In conclusion, g-C3N4 nanoparticle may be a potential photosensitizer for the treatment of staphylococcal biofilm-associated infections which are accessible to light.

کلیدواژه ها:

Photo-inactivation ، Wound infection ، Nanotechnology