Lipase Immobilization on Metal-Organic Framework and Evaluation of Initial Enzyme Concentration Effect

In the study, one of the metal-organic frameworks, MIL-101-NH2 (Cr), was synthesized by solvothermalmethod and functionalized with 2,4,6-trichloro-1,3,5-triazine (TCT). Amino MIL-101(Cr) and its chemicalfunctionalized form (TCT@NH2-MIL-101(Cr)) were used as support for candida rugose lipase (CRL) immobilization.The effect of initial CRL concentration on the enzyme loading and the immobilized enzyme activity were investigated.The result indicated that with increasing the amount of enzyme loading, the corresponding specific activity decreases.The amount of enzyme loading was determined by Bradford method. The catalytic activity of the immobilized lipasewas assayed by the hydrolysis of p-nitrophenyl palmitate (p-NPP). FT-IR spectra, XRD diffraction and FE-SEManalysis were used to confirm successive synthesis, functionalization and immobilization.

کلیدواژه ها:

Lipase ، Immobilization ، Metal-organic framework