The study of spermatogenesis effects of longum piper fruit on azoospermia Rat

Background: Couples have got 21% infertility problems in which 60% is due to male factor. Sometimes after cell migration to gonads, under the influence of various factors, gametogenesis does not happen. In addition to genetic factors, various factors cause infertility. In traditional medicine, azoospermia treatment is done with herbs.Methods: To investigate the spermatogenesis effect of longum piper fruit on azoospermia rats in this experimental study, was used 28 male white Wistar rats with 10 weeks old and weighing approximately 200±.10 grams. Rats were purchased from the Institut Pasteur and were kept under laboratory conditions. During the study, rats were in 12/12 hours of light- darkness, 24 ± 2 °C and room humidity was 55-60%. The 7 rats, normal group (Group 1), and then 21 rats were sterilized with 30mg/kg Busulfahe (Group2). After azoospermia assurance, Group 2 was divided into three subgroups with 7 rats: 1) Group 2-1: Control: Maintenance only at standard conditions. 2) Group 2-2: Sham, gavaging only with water. 3) Group 2-3: Treatment, gavage twice with longum piper fruit powder.Result: At the end of treatment, re-coloring spermatogenesis was examined in the testes. Lumen reduction in seminiferous tubules and reduction in mature sperm was significant and observed in control and Sham groups but in the group 2-3 dimensional analysis and epididymis was significantly reduced and mature spermatozoa was observed in seminiferous tubules. Spermatogenesis in the treatment group compared to the control group 48% improvement.Conclusion: In general, fruits and seeds the longum piper powder can result in azoospermia with spermatogenesis a dormant spermatogonia.

کلیدواژه ها:

Azoospermia ، Infertility ، Rat ، longum piper