The effect of two different combined antioxidants on the liquid storage of ram semen

Background: Oxidative damage to spermatozoa during storage is a potential cause of the decline in motility and fertility during hypothermic storage of liquid semen. During the recent years, the use of herbal antioxidants has attracted the attantion of several researchers. In this regards, it is of great interest to note that two-thirds of the world’s plant species have medicinal value; in particular, many medicinal plants have great antioxidant potential. The aim of this study was to determine the effects of the addition of the combined garlic extract (0, 1 and 1.5%) and stachys schtschegleevii (0, 4 and 6%) on quality of ghezel ram sperm during cooling preservationMethods: A total of 20 ejaculates were collected from four ram (five ejaculates from each ram) twice a week during the breeding season using an artificial vagina. Immediately after semen collection, the ejaculates were evaluated for sperm motility; only ejaculates with sperm motility over 80% were processed. The sperm motility, abnormal sperm, viability and the membrane integrity were determined during storage of semen at 4 ◦C for a period of 0, 6, 24 and 48 h of liquid storage, respectively.Result: We could not detect significant changes in total and progressive motility, abnormal sperm, viability and the membrane integrity.Conclusion: In conclusion, combined garlic extract and stachys schtschegleevii cannot improve the sperm quality during the liquid storage.

کلیدواژه ها:

Antioxidant ، Oxidative damage ، Sperm ، Liquid storage