Comparison of direct PCR and PCR using DNA extracted kit for determine tst and mecA genes in Staphylococcus aureus

PCR reactions one of the most accurate and most sensitive tests for the molecular assays.DNA ex-traction always is time-consuming and costly before the PCR process, which in somecases removed can be saving time and price. The aim of this study was to compare direct PCR and PCR with DNA extraction template for detection of tst,mec A and femA gens. in this study,clinical isolates of Staphylococcus aureus were confirmed by biochemical tests. Then with direct PCR by colony acquired of Muller Hinton agar culture and PCR with the extracted DNA extraction kit were studied for tst and mecA genes. For this test, three specific primers were used to target genes. amplification was observed of tst,mecA and femA gens with length 326bp,163bp and 132bp by direct PCR and PCR with the extracted DNA rele-vant to toxic shock syndrome toxin1 and resistant to methicilin. Finally, detected resistant strains and toxins strains by genes. Our results quantitatively similar each other and in therm of qualitatively were different. The results of PCR test with using extraction kit obtained the better quality. According to the results obtained in this study,to save time and cost in the detection of staphylococcus aureus can be used to direct PCR. Bands quality obtained in this way to PCR,DNA is acceptable and in some cases it can be used as a rapid diagnostic test. To make better quality must be used mineral oils

کلیدواژه ها:

Staphylococcus aurerous ، MRSA ، Direct PCR ، tst ، mecA