Study of vertical effect of aqueous-methanolic extract of Artemisia absinthium on erythromycin therapeutic dosage induced hepatotoxicity

Objective: Erythromycin is one of the macrolid antibiotics and its action is inhibition of protein synthesis of bacteria and bactericidal which occurs via binding to the 50s ribosomal RNA, but long term intake of erythromycin may cause hepatotoxicity. In this study, we evaluated erythromycin long term therapeutic dosage (100mg/kg) induced hepatotoxicity in gold fish and Artemisia absinthium protective effect. Method & Materials: The present study performed in 30 gold fish which divided in 3 group (n=10), at the first group we administered drug solvers as a placebo, and in the second group oral daily administration of therapeutic dose of erythromycin (100 mg/kg body weight) was given to gold fish, and in the third group aqueous-methanolic extract of Artemisia absinthium with dose of (500mg/kg body weight) was given 4 hours prior of administration of erythromycin(100mg/kg) for 21 days, (all drugs administered orally with gavages). Results & Conclusion: 3 weeks after bleeding from heart the serum was separated and the rate of ALT, AST, ALP, LDH, and Albumin activities were determined in the plasma of fish and results were recorded in special forms. At the end of experiment, it was found that long term use of erythromycin caused liver damage as was evident by statistically significant (P>o.05) increased in plasma activities of hepatic enzymes. Oral administration of aqueous-methanolic extract of Artemisia absinthium significantly prevented the occurrence of erythromycin induced liver damage. The increased level of serum enzymes such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), LDH and albumine observed in fish treated with erythromycin were very much reduced in fish treated with aqueous-methanolic extract of Artemisia absinthium. Results of this study revealed that Artemisia absinthium could afford a significant protection against erthromycin induced hepatocellular damage.

کلیدواژه ها:

Artemisia absinthium ، Erythromycin ، Antibiotic ، Hepatotoxicity ، Gold fish