Synthesize and functionalization of Mesoporous Silica Nanoparticles for drug delivery to cancer cells

Aim: Nowadays cancer is one of the main reasons for mortality, so scientists try to find new methods for drug delivery.1 Nanoparticles are promising carriers to overcome lots of problems in this area.2 Amongdifferent types of nanoparticles, Mesoporos Silica Nanoparticles (MSNs) show several attractive features for anticancer drug delivery.3 Methods: In this study we prepared MSNs with different functional groups including amine-modifiedMSNs, phosphonate-modified MSNs, PEGylated MSNs, MSNs functionalized with polyethyleneiminepolyethylene glycol (MSN-PEI-PEG) and unmodified MSNs by using co-condensation method. The nanoparticles were characterized with Fourier transform infrared spectroscopy (FTIR), transmissionelectron microscopy (TEM), BET surface area, particle size analyzer, Thermogravimetric Analysis (TGA) and Elemental Analysis (CHN). These nanoparticles were loaded with anticancer drug, epirubicin hydrochloride (EPI). Cytotoxicity of MSNs loaded with EPI were evaluated with MTT assay on C26cancer cell line and the in-vivo antitumor activity of MSNs loaded with EPI were evaluated in BALB/cmice bearing C26 colon carcinoma. Results: The TEM images show spherical and porous nanoparticles (Fig. 1). Phosphonated MSNs hadthe highest drug loading. Drug release from MSNs was pH- and time-dependent. In vitro drug release in Phosphate Buffer Saline with pH 7.4 was slow whereas in acetate buffer with pH 5.5 was rapid. In-vivo antitumor activity of MSN-PEI-PEG loaded with epirubicin was better than the other formulations. Conclusion: The results indicated that MSNs could be an effective nanocarriers for anti-tumor therapies

کلیدواژه ها:

Mesoporous silica ، Epirubicin ، Drug delivery ، Cytotoxicity ، Cancer ، Nanoparticle