Evolution of reduced graphene oxide modified carbon screen printed electrode for determination of buprenorphine

In this research, we have developed an electrochemical buprenorphine (BN) sensor based on reduced graphene oxide (r-GO) as an electrode modifier on the screen printed carbon electrode (SPCE) surface. BN oxidation pathway was proposed using voltammetric studies and with theaid of Density Functional Theory (DFT). The effects of various parameters including pH, modifier amount, accumulation time, and accumulation potential on the oxidation peak current ofBN were optimized. Under the optimized conditions, the linear response range and the limit of detection were obtained as 0.50–100 μM and 0.16 μM (S/N=3) using differential pulse voltammetry (DPV), respectively. The selectivity of the modified electrode in the presence ofpotential interferences was also studied, and finally the electrode was. successfully employed for the determination of BN in spiked urine samples.