Measurement of lipid peroxidation and protein oxidation markers in plasma of Alzheimer's disease patients

Introduction: Studies have shown oxidative stress (OS) is an important indicator in the pathogenesis of Alzheimer's disease (AD), but some studies regarding OS have controversial results. Therefore, in this study, we evaluated the plasma levels of Malondialdehyde (MDA) and carbonyl as lipid and protein oxidative damage markers in AD patients and control group. Materials and methods: This study was carried out on 30 AD patients with severe probable AD and 30 sex and age matched healthy subjects. Alzheimer's patient diagnosis based on National Institute of Neurological and Communicative Disorders and Stroke and the Alzheimer's Disease and Related Disorders Association (NINCDS/ADRDA) criteria. Also for patients, the Mini-Mental State Examination (MMSE), computed tomography (CT) scan and brain magnetic resonance imaging (MRI) were done. The plasma levels of Malondialdehyde (MDA) and carbonyl were measured by spectrophotometric methods. Results: MMSE score for AD and control groups were achieved 3.6 ± 0.24 and 28.3 ± 0.77 respectively (p<0.001). Plasma level of MDA significantly increased in AD patients in comparison to control group (p=0.036), the plasma level of carbonyl increased in patients but was not significant (p=0.117). Also Area under ROC curve (AUC) for MDA and carbonyl were 0.63 and 0.61, respectively. Conclusion: Our results confirm the link between damage caused by oxidative stress and AD and these markers may be contributed in etiology of AD. Thus, it is recommended to determination of the oxidative stress markers in AD patients for more effective treatment of patients.

کلیدواژه ها:

Alzheimer's disease ، Oxidative stress ، MDA ، Carbonyl