Bioremediation of DSO contaminated soil in laboratory scale

Six strains isolated from DSO (Di-Sulfide Oil) contaminated soils. Among them, two strains had high potential to remove DSO from contaminated soil. By preliminary tests these strains identified as Pacnibacillus and Rhodococcus that both of them were gram positive bacteria. The Taguchi method was adopted to determine the optimal condition of DSO removal from contaminated soil. Rhodococcus remediated DSO from soil by 4%. Bioremediation of DSO in soil was investigated by gas chromatography and UV-VIS absorption spectroscopy techniques. The results showed that addition of water (20μl/gr) to soil is necessary for DSO removal by both strains and none of the strains could survive in DSO concentrations more than 30μl/gr.