Genetic Diversity of Phytophthora Sojae in Iran

Objective: The aim of this study was to estimate genetic diversity and morphological relationships of Phytophthora sojae from Iran. Methods: During 20052007, 142 isolates of P. sojae were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of fifty isolates of P. sojae was amplified with 20 RAPD and 5 ISSR primers. Results:Five primers amplified 77 fragments, of which 20 were polymorphic, indicating low variability. RAPD and ISSR primers clustered isolates into three distinct groups at 85% similarity. Genetic diversity among isolates detected intra specific variability even among isolates of the same geographic origin. There is no correlation between races, geographical distribution, and molecular markers

کلیدواژه ها:

Soybean ، Root rot ، RAPD ، ISSR ، Glycine max ،