Polymorphism of 5′ flanking region of lactoferrin gene in khuzestan buffaloes

سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 686

فایل این مقاله در 6 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJABBR-1-7_009

تاریخ نمایه سازی: 26 اسفند 1394

چکیده مقاله:

Lactoferrin is a glycoprotein with molecular weight 80 kDa iron-binding bond, which is composed of 690 amino acids. In most mammalian body fluids such as sweat, semen, tears, and saliva and milk neutrophil granules there. bovine lactoferrin gene be associated with susceptibility/resistance to mastitis and even with some economically important production traits. This study was carried out to detect polymorphism in lactoferrin gene in 85 Bufallo to 4 location khuzestan province were selected randomly. After extracting DNA, amplification of 367 bp of lactoferrin gene was performed using specific primers. In this research four SSCP pattern for AA, BB, EE and FF lactoferrin gene in Bufallo were found in KhuzestanProvince. The results showed that lactoferrin gene is so polymorphism in studied population. Gene frequencies for A, B, E and F alleles were 0.341, 0.259, 0.118 and 0.282 respectively. Therefore, the results confirmed that SSCP technique can be used to identify different genotypic variation in these breeds and is a useful tool for selection programs based on marker-assisted selection

نویسندگان

Maryam Khatibi

MSc student, associate professor and assistant professor, Ramin University of agriculture and Natural Resources, Ahwaz, Iran

Hedayatullah Roshanfekr

MSc student, associate professor and assistant professor, Ramin University of agriculture and Natural Resources, Ahwaz, Iran

jamal Fayazi

MSc student, associate professor and assistant professor, Ramin University of agriculture and Natural Resources, Ahwaz, Iran

Khalil Mirzade

MSc student, associate professor and assistant professor, Ramin University of agriculture and Natural Resources, Ahwaz, Iran