The effect of growth regulators (NAA and 2,4-D) on callus induction in citrus rootstock

سال انتشار: 1394
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 618

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شناسه ملی سند علمی:

ASECONF01_052

تاریخ نمایه سازی: 30 بهمن 1394

چکیده مقاله:

The present investigation was undertaken to study the effect of different concentrations and combinations of 2, 4-dichloro-phenoxyacetic acid (2, 4-D), naphthalene acetic acid (NAA) on callus induction of five citrus rootstocks (Sour orange, Citromelo, Citrange, Poncirus, Volkameriana) on MS basal medium. Different concentration of 2,4-D and NAA were tasted in order to obtain the best callus formation. Maximum callus induction response (100%) was obtained on MS medium with 2,4-D (2 and 3mg/l) and NAA (1.5 mg/l) for Volkameriana. Best callus induction response of Citrange (83.33%) was observed on MS medium with 2,4-D (1 mg/l) and NAA (1.5 mg/l). The highest frequency of the callus induction rate for Poncirus and Citromelo (91.67%) was occurred on MS medium supplemented with 2.4-D (3 mg/l) and NAA (1.5 mg/l). MS medium supplemented with 2,4-D (1 mg/l) and NAA (1.5 mg/l) showed maximum callus induction (91.76%) for Sour orange.

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نویسندگان

Seyedeh Zahra Hosseini

PhD. Student, Sari Agricultural Sciences and Natural Resources University

Nadali Babaeian Jelodar

Professor, Sari Agricultural Sciences and Natural Resources University

سام Rahimian

Professor, Sari Agricultural Sciences and Natural Resources University

Gholamali Ranjbar

Professor, Sari Agricultural Sciences and Natural Resources University