The response of embryo explant to tissue culture in wheat varieties

Callus induction of three wheat varieties Sardari 101, were assessed by mature embryo in MS and B5 media with three levels of 2,4-D. Analysis of variance indicated that variety effect was significant on callus induction frequency, callus size and callus fresh weight. The best callus induction condition and callus size was obtained in 2 mg/l 2,4-D and the highest callus induction frequency was in MS medium.