Comparative performance of the anion exchange and dye-ligand recovery of intracellular enzymes from unclarified feed stocks

Development of a rapid and simplified primary capture step (i.e. fluidised/expanded bed adsorption) for the direct selective recovery of intracellular enzymes from particulate-containing yeast disruptate has been undertaken. The Kiesleguhr-agarose composite adsorbent derivatised with Cibacron Blue 3GA was used in batch binding experiments as well as in commercial and custom assembled fluidised/expanded bed contactors and the results was compared with the performance of the anion exchange adsorbent (Streamline QXL). Use of the pseudo-affinity has enabled the enzyme to be more selectively captured, indicating that there was less interference of negatively charged components present in feedstock. Additionally, the better adsorption performance achieved attributed to the fact that dye-ligand was less sensitive to the ionic strength of feedstocks . The generic application of such techniques for the

کلیدواژه ها:

Dye-ligand chromatography ، intracellular enzymes ، Expanded bed adsorption ، yeast bioprocessing ، specific interaction