MitoQ and Mito-TEMPO Reduce Lipid Peroxidation and Improve Fertility of Cooled Rooster Sperm

This study evaluated the effects of mitochondria-targeted antioxidants (MitoQ and Mito-TEMPO) compared to untargeted antioxidants (SOD and CoQ۱۰) on viability, lipid peroxidation, membrane integrity, and fertility of cooled rooster sperm (۴°C for ۴۸ hours). Semen samples were collected from Ross-۳۰۸ roosters and divided into eight groups: control, SOD (۱۰۰ U/mL), CoQ۱۰ (۱ μM), MitoQ (۵۰ and ۱۰۰ nM), Mito-TEMPO (۵۰ and ۱۰۰ μM), and MitoQ+Mito-TEMPO combination. Viability was assessed by eosin-nigrosin staining, malondialdehyde (MDA) concentration by TBA reaction, and membrane integrity by hypoosmotic swelling test (HOST). After ۲۴ hours of cooling, MQ۱۰۰, MT۱۰۰, and MQ/MT groups were used for artificial insemination of hens (۱۰ hens/group, twice weekly for one month). Results showed that at ۲۴ and ۴۸ hours, mitochondria-targeted groups exhibited the highest viability and membrane integrity and the lowest MDA concentrations (P<۰.۰۵). Fertility rates in MQ۱۰۰ (۵۸.۳%), MT۱۰۰ (۵۹.۸%), and MQ/MT (۶۵.۱%) were significantly higher than control (۴۱.۶%). Hatchability did not differ significantly. Mitochondria-targeted antioxidants are recommended for preserving fertility of cooled rooster sperm.

کلیدواژه ها:

Rooster ، Cooled semen ، MitoQ ، Mito-TEMPO ، Lipid peroxidation ، Fertility ، Membrane integrity