Eriobotrya japonica L. Seed Polysaccharide Extract: Extraction, Characterization, and Biological Activities

The present study focused on extracting Loquat Seed Polysaccharides (LSP) using an ultrasonic-assisted method and evaluating their structural and functional properties. The extraction was optimized using response surface methodology, leading to a maximum yield of ۲۹.۰۸% under the following optimum conditions: ultrasonic power of ۱۲۰ W, water-to-raw material ratio of ۲۰ mL/g, and sonication for ۲۵ min. The purified LSP showed an extraction yield of ۸۳.۲۹%. According to gel permeation chromatography, the molecular weight of LSP was determined at ۱۳۳.۸۹ kDa. High-performance liquid chromatography results indicated that the LSP was primarily composed of rhamnose, arabinose, and glucose. Confirmation of β-configurations in LSP was done using Fourier Transform InfraRed (FT-IR) spectroscopy and Nuclear Magnetic Resonance (NMR) analysis. The OH and DPPH radical scavenging abilities of LSP were concentration dependent, with values of ۷۸.۷۵% and ۶۶.۷۶%, respectively, at ۰.۵ mg/mL concentration. This polysaccharide extract showed in vitro cytotoxicity effects against HeLa cells. LSP might be a candidate as a natural component in the pharmaceutical and functional food industries based on its anti-cancer activities and antioxidant properties.

کلیدواژه ها:

Antioxidant activity ، Structural characterization ، Functional properties ، polysaccharide extract ، Loquat ، Response surface methodology