Interaction effect of thiamine (Vit B۱) and ۲۴-epibrassinolide on rapeseed (Brassica napus) growth improvement and change of some biochemical parameters content under cadmium stress

The heavy metal cadmium is an environmental pollutant that causes various toxicities in living organisms, especially plants. The organic substances thiamine and ۲۴-epibrassinolide are known as plant growth regulators that cause different growth and physiological responses in plants. There have been no studies on the interaction of thiamine and epibrassinolide with heavy metals. In this study, the induction of possible resistance-inducing effects by the application of thiamine and epibrassinolide treatment on the reduction of cadmium toxicity in rapeseed was investigated. Rapeseed plants were grown in a greenhouse environment under standard conditions for ۴ weeks. After this period, the effects of experimental treatments including concentrations of thiamine (۰, ۱۰۰, and ۲۰۰ μM), epibrassinolide (۰, ۰.۰۲, and ۰.۵ μM), and cadmium (۰, ۲۵۰, and ۵۰۰ μM) were tested on plants every other day for ۷ days. The experiment and treatments were performed in a completely randomized design with ۳ replications on the plants. The resulting data were statistically analyzed using ANOVA and Duncan's multiple range test at a significance level of ۵% using SPSS ۱۸.۰ software. The results of this study showed that cadmium stress reduced plant height parameters, fresh and dry weights of shoots and roots, and reduced the content of photosynthetic pigments and sugars in the leaves of stressed plants compared to the control.

کلیدواژه ها:

Anthocyanin ، Flavonoids ، Heavy metal ، environmental pollutant ، Phenolic Compounds