Effect of priming using some growth regulators on germination indices and biochemical traits of triticale (x Triticosecale) seeds under aging

This study was conducted in ۲۰۲۳ to investigate the effect of priming with some growth regulators on the germination indices and biochemical traits of triticale seeds under accelerated aging. The experiment was arranged as a factorial based on a completely randomized design with three replicates. It was carried out at the Seed Science and Technology Laboratory, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili. The treatments included three levels of aging (۹۵%, ۸۵%, and ۷۵%) and six types of priming (control, hydro-priming, priming with cytokinins, priming with spermidine, priming with salicylic acid, and priming with auxin). Seeds were also primed by soaking for ۲۴ hours at ۲۵ °C in distilled water and hormone solutions (the hormones were used at a concentration of ۱ mM for ۲۴ hours, based on preliminary trial results). In the preliminary trial, concentrations of ۰.۲۵, ۰.۵, ۱, ۱.۵, and ۲ mM were tested. The results indicated that aging reduced the germination percentage; however, the types of priming, particularly priming with auxin, mitigated the effects of aging and improved this trait. With increasing aging, the amount of seed reserves utilized, utilization efficiency of reserves, total phenol content, flavonoids, total fatty acids, and anthocyanins decreased, while free sugars, glucose, electrical conductivity, and ion leakage increased. Seed reserve utilization, flavonoid content, and anthocyanin levels in the auxin priming treatment increased by approximately ۲۲%, ۱۳.۷%, and ۷۳.۶%, respectively, compared to the control. Ion leakage and soluble sugar content in auxin priming at ۷۵% aging decreased by ۵۱% and ۸۴%, respectively, compared to the control. The results of this study indicate that seed priming, especially with auxin, can enhance aged triticale seeds and improve germination; thus, this approach may serve as an effective strategy to enhance the viability of aged seeds.

کلیدواژه ها:

Auxin ، Priming ، Exhaustion ، Free sugars ، Glucose ، اکسین ، پرایمینگ ، فرسودگی ، قندهای آزاد ، گلوگز